pCDF1-MCS2-EF1-copGFP慢病毒载体

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载体订购信息

产品编号
GS-1483
产品名称
pCDF1-MCS2-EF1-copGFP 慢病毒载体
市场价格
￥ 1400
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载体基本信息

载体名称:
pCDF1-MCS2-EF1-copGFP
载体类型:
慢病毒载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
6771 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
LNCX: AGCTCGTTTAGTGAACCGTCAGATC
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

The pCDF1-MCS2-EF1-copGFP Vector contains the full-length copGFP gene with optimized human codons for high level of expression of the fluorescent protein from the CMV promoter in mammalian cells. The copGFP marker is a novel natural green monomeric GFP-like protein from copepod (Pontellina sp.). The copGFP protein is a non-toxic, non-aggregating protein with fast protein maturation, high stability at a wide range of pH (pH 4-12), and does not require any additional cofactors or substrates. The copGFP protein has very bright fluorescence that exceeds at least 1.3 times the brightness of EGFP, the widely used Aequorea victoria GFP mutant. The copGFP protein emits green fluorescence with the following characteristics:

emission wavelength max – 502 nm;
excitation wavelength max – 482 nm;
quantum yield – 0.6;
extinction coefficient – 70,000 M-1 cm-1

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