pcDNA3.1/His A哺乳动物表达载体

Read More
Thumbnail image

载体订购信息

产品编号
GS-1974
产品名称
pcDNA3.1/His A 哺乳动物表达载体
市场价格
￥ 1000
沟通洽谈

载体基本信息

载体名称:
pcDNA3.1/His A
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin (G418)
载体大小:
5514 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
测序引物3’:
pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
报告基因:
/

载体图谱展示

载体图谱

载体说明

pcDNA3.1/His A, B, and C are 5.5 kb vectors derived from pcDNA3.1 and designed for high-level expression and purification of recombinant proteins in mammalian hosts. The vectors are supplied in three reading frames to facilitate in frame cloning with a polyhistidine metal-binding tag. The human cytomegalovirus (CMV) immediate-early promoter provides high-level expression in a wide range of mammalian cells. In addition, the vector replicates episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/His lacZ, is the pcDNA3.1/His B vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the N-terminal peptide. pcDNA3.1/His lacZ is included for use as a positive control for transfection, expression, and purification in the cell line of choice.

关于我们 About Us

公司简介
项目起源

基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。

团队使命

让基因研究更简单!

团队愿景

您值得信赖的一站式基因研究合作伙伴!

联系我们