pFastBac1昆虫细胞表达载体

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载体订购信息

产品编号
GS-1166
产品名称
pFastBac1 昆虫细胞表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pFastBac1
载体类型:
昆虫细胞表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Gentamicin (庆大霉素)
载体大小:
4776 bp 点击查看完整序列
启动子:
Polyhedrin
测序引物5’:
pFastbac-F: TATTCCGGATTATTCATACC
测序引物3’:
pFastBac-R: ACAAATGTGGTATGGCTGA
报告基因:
/

载体图谱展示

载体图谱

载体说明

The Bac-to-Bac? Vector Kit contains a pFastBac? 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac? Baculovirus Expression System (Cat. No. 10359-016), which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac? System relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells (Figure 1). This system features:

  • Time-saving expression bacmid. With Bac-to-Bac?, the expression cassette of the pFastBac? vector recombines with the parent bacmid in DH10Bac? E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.
  • Easy colony screening. The parent bacmid in DH10Bac? E. coli contains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.

The Bac-to-Bac? Baculovirus Expression System is designed for fast, small-scale production of recombinant baculovirus. The pFastBac? 1 vector provided by this kit offers the strong polyhedrin promoter for protein expression and a large multiple cloning site for simplified cloning.

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