pGL4.18[luc2P/Neo]信号通路报告载体

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载体订购信息

产品编号
GS-1732
产品名称
pGL4.18[luc2P/Neo] 信号通路报告载体
市场价格
询价
沟通洽谈

载体基本信息

载体名称:
pGL4.18[luc2P/Neo]
载体类型:
信号通路报告载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin (新霉素)
载体大小:
5727 bp 点击查看完整序列
启动子:
/
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱载体图谱

载体说明

The pGL4.18[luc2P/Neo] Vector(a–e)encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is also engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression.

The pGL4.18[luc2P/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with a greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.

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