pGL4.27[luc2P/minP/Hygro]信号通路报告载体

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载体订购信息

产品编号
GS-1562
产品名称
pGL4.27[luc2P/minP/Hygro] 信号通路报告载体
市场价格
询价
沟通洽谈

载体基本信息

载体名称:
pGL4.27[luc2P/minP/Hygro]
载体类型:
信号通路报告载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
6010 bp 点击查看完整序列
启动子:
/
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱载体图谱

载体说明

The pGL4.27[luc2P/minP/Hygro] Vector(a–f)encodes the luciferase reporter gene luc2P and is designed for high expression and reduced anomalous transcription. The vector contains a multiple cloning region for insertion of a response element of interest upstream of a minimal promoter and the luc2P gene. luc2P is a synthetically derived luciferase sequence with humanized codon optimization. The luc2P gene also contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow for selection in E. coliand a mammalian selectable marker for hygromycin resistance. See the pGL4 Luciferase Reporter Vectors Technical Manual #TM259 for more information.

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