载体订购信息

产品编号
GS-1238
产品名称
pRSFDuet-1 原核表达载体
市场价格
¥ 1000
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载体基本信息

载体名称:
pRSFDuet-1
载体类型:
原核表达载体
克隆方法:
/
拷贝类型:
载体抗性:
Kanamycin (卡那霉素)
筛选标记:
/
载体大小:
3829 bp 点击查看完整序列
启动子:
/
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the RSF1030-derived RSF replicon, lacI gene and kanamycin resistance gene (KnR). This vector can be used in combination with pACYCDuet?-1 (Cat. No. 71147-3), pCDFDuet?-1 (Cat. Np. 71340-3), and pETDuet?-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.

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