pTet-Off-Advanced四环素调控系统

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载体订购信息

产品编号
GS-1722
产品名称
pTet-Off-Advanced 四环素调控系统
市场价格
￥ 1500
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载体基本信息

载体名称:
pTet-Off-Advanced
载体类型:
四环素调控系统
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin (新霉素)
载体大小:
7140 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

pTet-Off-Advanced expresses an improved version of the Tet (tetracycline)-controlled transactivator protein called tTA-Advanced (1–4). It is expressed to higher levels than the original tTA used in the Tet-Off? System (2). The tTA-Advanced protein is a fusion of amino acids 1–207 of the Tet repressor (TetR) and 39 amino acids containing three minimal "F"-type transcriptional activation domains (ADs) from the VP16 protein of herpes simplex virus. The gene is fully synthetic, lacks cryptic splice sites in the mRNA, and utilizes human codon preferences for stable expression in mammalian cells.

载体应用

The pTet-Off-Advanced Vector is used to develop stable Tet-Off Advanced cell lines, which are hosts for a tetracycline-controlled gene expression system. Once a vector containing a gene of interest under the control of a Tet-responsive element (e.g., TRE-Tight or TRE2) is transfected into a Tet-Off Advanced stable cell line, tTA-Advanced binds to the TRE in the absence of doxycycline (Dox, a tetracycline derivative), and activates transcription of the gene of interest to a very high level. In the presence of Dox, tTA-Advanced is unable to bind the TRE in a Tet-responsive promoter and the system is inactive. Additional information on TRE-containing vectors and protocols describing how to construct a Tet-Off Advanced cell line can be found in the Tet-Off Advanced Inducible Gene Expression System User Manual (PT3945-1).

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