pTet-On四环素调控系统

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载体订购信息

产品编号
GS-1602
产品名称
pTet-On 四环素调控系统
市场价格
￥ 1000
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载体基本信息

载体名称:
pTet-On
载体类型:
四环素调控系统
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin (新霉素)
载体大小:
7400 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

pTet-On? expresses the reverse tet-responsive transcriptional activator (rtTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-On is similar to pTet-Off except for four amino acid changes that convert the TetR to a rTetR, and consequently, tTA to rtTA. (pTet-Off also contains several silent mutations.) pTet-On was originally described as pUHD17-1neo by Gossen et al. (1995). pTet-On can be distinguished from pTet-Off by digestion with Hind III.

载体应用

The pTet-On Vector is used to develop stable Tet-On cell lines. After a vector that contains a gene under the control of a tet-responsive element (TRE) is transfected into a Tet-On cell line, the rtTA binds to the TRE, thus activating transcription in the presence of doxycycline (Dox). As Dox is removed from the culture medium, transcription from the TRE is turned off in a highly dose-dependent manner. Additional information on TRE-containing vectors and protocols describing the construction of Tet-On cell lines can be found in the Tet Systems User Manual (PT3001-1).

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