pcDNA4/TO/myc-His A四环素调控系统

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载体订购信息

产品编号
GS-1595
产品名称
pcDNA4/TO/myc-His A 四环素调控系统
市场价格
￥ 1000
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载体基本信息

载体名称:
pcDNA4/TO/myc-His A
载体类型:
四环素调控系统
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Zeocin
载体大小:
5151 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
CMV-F; BGHrev
测序引物3’:
/
报告基因:
/

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载体说明

A Tetracycline-Regulated Expression System without Viral Transactivators
The T-REx? System yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2).

Specific Activation
The T-REx? System uses a repressor mechanism that blocks transcription from the powerful CMV promoter in the absence of tetracycline. Because the T-REx? System elements do not use viral transactivators, you can achieve high-level expression from the complete CMV promoter without secondary, non-specific activation of host genes.

The T-REx? Mechanism
The T-REx? transcriptional control elements are illustrated in Figure 1. Two tetracycline operator sequences (TetO2) have been inserted between the TATA box of the CMV promoter and the

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